Due to the coronavirus outbreak worldwide, global demand for some personal protective equipment (PPE) is exceeding supply. In addition, manufacturing of PPE and many other wound care and infection prevention products have been impacted by global response to coronavirus. Gram's decolorizer, a mixture of ethyl alcohol and acetone, is then added. This is the differential step. Gram-positive bacteria retain the crystal violet-iodine complex while gram-negative are decolorized. 4. Finally, the counterstain safranin (also a basic dye) is applied. Since the gram-positive bacteria are already stained purple, they are.
The slide is again washed in water and then decolorized in absolute ethyl alcohol or acetone. A mixture of ecetone-ethyl alcohol (1:1) can also be used for decolorization. The process of decolorization is fairly quick and should not exceed 30 seconds for thin smears. Acetone is a potent decolorizer and when used alone can decolorize the smear.
EDM3 (formerly HealthLink) offers a full line of Stains and Reagents for your laboratory needs. Whether you need Monsel's Solution, Gram Stain, Wright's Stain, Dichloracetic Acid, Potassium Hydroxide or Acetone, EDM3 probably carries it. We have over 300 items in our Stains & Reagents line - if we don't carry it, we can custom formulate.
Trading name: GRAM DECOLORIZER SOLUTION 3 Chemical name: - Catalogue number: GD3-OT-X** ... - ≥ 50 % acetone Flam. Liq. 2; H225 Eye Irrit. 2; H319 STOT SE 3; H336 SECTION 4. First aid measures ... water spray or alcohol resistant foam Unsuitable extinguishing media: No information available..
Acetone:Alcohol (1:1) for decolorizing, for Gram staining. Supplier: Ricca Chemical. Danger. Acetone-Alcohol, 1 + 1 Decolorizer Solution for use in Gram Staining. Flash Pt: –18 °C.